- Medium for general purposes, recommended for molecular genetics studies with E. coli.
Suspend 35.0 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45- 50º C, mix well and dispense into plates.
- Soy Peptone 10
- Yeast Extract 5
- Sodium Chloride 5
- Bacteriological Agar 15