Product Information
Product name: Live and Dead Cell Double Staining Kit
Application notes:
Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes LiveDye, a cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and NucleiDye, a cell non-permeable red fluorescent dye (Ex/Em = 535/617), to stain dead cells.
 
Fig.1(Above first picture). Hela cells stained with Live and Dead Cell Double Staining Kit. A: Live cells stain green, B: Dead cells (30min incubation in 70% ethanol) stain red.
Fig.2(Above second picture). Hela cells incubated with staining solution in Live and Dead Cell Double Staining Kit for 20min.
Product Properties
Kit components
• LiveDye
• NucleiDye
• Assay Buffer (10X)
Features & Benefits
• Suitable for mammalian cells staining.
• Useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy.
• LiveDye (green, Ex/Em=488/530nm), NucleiDye (red, Ex/Em= 535/617nm).
Usage notes
NucleiDye is a potential mutagen. Use appropriate precautions when handling this reagent.
Storage instructions
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.