- For E.coli in molecular genetics studies
Suspend 40 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC, mix well and dispense into plates. Store at 4°C. The color of the prepared medium is clear amber, slightly opalescent.
- Tryptone (pancreatic digest of casein) 10
- Sodium Chloride 10
- Yeast Extract 5
- Bacteriological Agar 15