- Medium used with glycerol to cultivate recombinant strains of E.coli.
Suspend 47,6 grams of the medium in 900 ml of distilled water .Mix well. Add 4 ml of glycerol and adjust to a final volume of 1000 ml. and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Once prepared, store at 2 - 8ºC.
- Tryptone (Pancreatic digest of casein): 12
- Yeast extract 24
- Monopotassium phosphate 2.2
- Dipotassium phospate 9.4