Product Information
Product name: Normal Rat Serum
Host: Rat
Applications: BL, ELISA, ICC, IF, IHC
Application notes:
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).
Preparation method:
Normal rat serum is processed from blood collected from non-immunized normal adult rats. Normal serum diluted to 5%-20% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. 2ml, 10ml package sizes are available, whicle bulk size available upon request.
Purification: 
Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2
Purity: Whole serum
 
Fig(Please see above). Normal rat serum is processed from blood collected from non-immunized normal adult rats. Normal serum diluted to 5%-20% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. 2ml, 10ml package sizes are available, whicle bulk size available upon request.
 
Product Properties
Formulation: Liquid solution
Storage buffer: 10 mM sodium phosphate, 0.15 M sodium chloride with 0.05% sodium azide as preservative, pH 7.2
Storage instructions:
Store at 2-8°C or aliquot and store at or below -20°C for long-term storage. Avoid repeated freezing and thawing.
Shipping:  Gel pack with blue ice.
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
 
Background
Normal rat serums, which are from non-immuned rat hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5%-20% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.