- Medium used with glycerol for the cultivation of recombinant strains of E.coli
Suspend 50.8 grams of the medium in 900 ml of distilled water .Mix well. Add 4 ml of glycerol and adjust to a final volume of 1000 ml. and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Once prepared, store at 2 - 8ºC.
- Yeast Extract 24
- Tryptone 12
- Dipotassium Phosphate 12.5
- Monopotassium Phosphate 2.3