- For the cultivation of a wide range of fastidious microorganisms, particulary Brucella from clinical samples
Suspend 26 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121ºC for 15 minutes.
- Tryptose 20
- Sodium Chloride 5
- Dextrose 1
- Thimine Chlorohydrate 0.005