- For the differentiation of Enterobacteria, particularly Proteus from Salmonella and Shigella from clinical samples
Suspend 3.87 grams of the medium in 100 ml of distilled water without heating. When the powder is dissolved, sterilize by filtration. Dispense quantities of 0.5 to 2 ml in small sterile tubes. Larger volumes can be used but the reactions will be slower. Do not sterilize in autoclave. Do not boil the medium.
When there is no filter available, the medium can be sterilized at 100 -110°C for 10 minutes. If the medium is prepared and inoculated immediately it provides good results without sterilizing.
- Urea 20
- Dipotassium Phosphate 9.5
- Monopotassium Phosphate 9.1
- Yeast Extract 0.1
- Phenol Red 0.01