- For yeast classification based on amino acids and carbohydrates requirements
Prepare a 10X solution by dissolving 6.7 grams of the medium in 100 ml of distilled water with 5 grams of dextrose, or the equivalent amount of another carbohydrate, and 5 - 10 mg of the desired amino acid. Mix well. Heat with frequent agitation until complete dissolution. DO NOT BOIL. DO NOT AUTOCLAVE. Sterilize the solution by filtration. Store at 4°C.
Prepare the final medium by aseptically pipetting 0.5 ml of the 10X solution to 4.5 ml of distilled water. Swirl to mix solution before inoculation. The color of the prepared medium is greenish yellow.
- Ammonium Sulfate 5
- Yeast Nitrogen Base 1.7