- For maintaining and developing yeast in molecular microbiology procedures
Suspend 45 grams of the dehydrated medium in 900 ml of distilled water. Heat with frequent agitation until complete dissolution.
Sterilize in the autoclave at 121°C for 15 minutes. Cool to 55°C and add 100 ml of sterile 20% dextrose solution under sterile conditions. Mix well and pour into Petri dishes. Store at 4°C. The color of the prepared medium is amber.
- Peptone 20
- Yeast Extract 10
- Bacteriological Agar 15