Reverse Transcriptases​

M-MLV Reverse Transcriptase (reconbinant)

Size: (100 μl, 98,000원), (200 μl, 179,000원)

Contents

  • Size(100 μl):  Size: 5,000 U, M-MLV: 100 μl, 5xFirst-Strand Buffer: 100 μl

  • Size(200 μl):  Size: 10,000 U, M-MLV: 200 μl, 5xFirst-Strand Buffer: 100 μl

Description

Our offered M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Dongsheng M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Source

E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Application

Generation of first strand cDNA for use in:
-PCR, see Protocol for First-strand cDNA Synthesis;
-real-time PCR;
-second strand cDNA synthesis;
DNA labeling;
Analysis of RNA by primer extension.

Quality Control

The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.

Functionally tested in first strand cDNA synthesis.

Store Buffer

20m M Tris-HCl (pH 7.5)
200 mM NaCl
0.1 mM EDTA
1 mM DTT
0.01%  NP-40
50%  glycerol

5xFirst Strand Buffer

250 mM Tris-HCl (pH 8.3 at 25℃)
375 mM KCl
15 mM MgCl2
50 mM DTT

Médico™ RT-PCR Kit

Size: (20 rxn, 150,000원), (100 rxn, 문의) 메뉴얼 다운받기>>

Contents

  • Size(20 rxn): M-MLV(200U/μl,): 20 μl, RNasin (40U/μl): 12 μl, Oligo(dT)15 (50 μM): 20 μl, Random hexamer primer (50 μM): 20 μl, 5xFirst-Strand Buffer: 80 μl, RNase-free ddH2Ol 1mL, dNTPs(10mM each): 50 μl

  • Size(100 rxn):  M-MLV(200U/μl,): 100 μl, RNasin (40U/μl): 60 μl, Oligo(dT)15 (50 μM): 100 μl, Random hexamer primer (50 μM): 100 μl, 5xFirst-Strand Buffer: 400 μl, RNase-free ddH2Ol 1mLx 5, dNTPs(10mM each): 250 μl

Storage

All components of the kit should be stored at -20°C. Keep control RNA at -70°C for longer storage.

Description

RT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The RT-pcr kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. You get all of the components you need for successful first-strand cDNA synthesis, saving your time and ensuring your success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.

 

Application

First strand cDNA synthesis for RT-PCR .
Construction of cDNA libraries.
One-step RT-PCR
Primer extension

RNA Products

RNA Products

Water, DEPC Treated (DNase, RNase Free)

Size: 500ml 40,000원

*주문생산가능

Description:

Purified by ion exchange, carbon extraction, glass distillation and filtration

Application:

For use with RNA

MEDICO   

Tel : 070-8615-5557  Fax : 0504-981-5578

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