M-MLV Reverse Transcriptase (reconbinant)
Size: (100 μl, 98,000원), (200 μl, 179,000원)
Size(100 μl): Size: 5,000 U, M-MLV: 100 μl, 5xFirst-Strand Buffer: 100 μl
Size(200 μl): Size: 10,000 U, M-MLV: 200 μl, 5xFirst-Strand Buffer: 100 μl
Our offered M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Dongsheng M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase.
E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Generation of first strand cDNA for use in:
-PCR, see Protocol for First-strand cDNA Synthesis;
-second strand cDNA synthesis;
Analysis of RNA by primer extension.
The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.
Functionally tested in first strand cDNA synthesis.
20m M Tris-HCl (pH 7.5)
200 mM NaCl
0.1 mM EDTA
1 mM DTT
5xFirst Strand Buffer
250 mM Tris-HCl (pH 8.3 at 25℃)
375 mM KCl
15 mM MgCl2
50 mM DTT
Médico™ RT-PCR Kit
Size: (20 rxn, 150,000원), (100 rxn, 문의) 메뉴얼 다운받기>>
Size(20 rxn): M-MLV(200U/μl,): 20 μl, RNasin (40U/μl): 12 μl, Oligo(dT)15 (50 μM): 20 μl, Random hexamer primer (50 μM): 20 μl, 5xFirst-Strand Buffer: 80 μl, RNase-free ddH2Ol 1mL, dNTPs(10mM each): 50 μl
Size(100 rxn): M-MLV(200U/μl,): 100 μl, RNasin (40U/μl): 60 μl, Oligo(dT)15 (50 μM): 100 μl, Random hexamer primer (50 μM): 100 μl, 5xFirst-Strand Buffer: 400 μl, RNase-free ddH2Ol 1mLx 5, dNTPs(10mM each): 250 μl
All components of the kit should be stored at -20°C. Keep control RNA at -70°C for longer storage.
RT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The RT-pcr kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. You get all of the components you need for successful first-strand cDNA synthesis, saving your time and ensuring your success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.
First strand cDNA synthesis for RT-PCR .
Construction of cDNA libraries.